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Literature summary for 1.5.3.17 extracted from

  • Keinaenen, T.A.; Grigorenko, N.; Khomutov, A.R.; Huang, Q.; Uimari, A.; Alhonen, L.; Hyvoenen, M.T.; Vepsaelaeinen, J.
    Controlling the regioselectivity and stereospecificity of FAD-dependent polyamine oxidases with the use of amine-attached guide molecules as conformational modulators (2018), Biosci. Rep., 38, BSR20180527 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.5.3.17

Application

Application Comment Organism
synthesis application of simple guide molecules, being either covalently attached to polyamines or used as a supplement to the substrate mixtures, for controlling the enzyme's regioselectivity and stereospecificity Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.061
-
 (3S,3'S)-dimethylspermine pH 7.4, 25°C Saccharomyces cerevisiae
0.077
-
 spermine pH 7.4, 25°C Saccharomyces cerevisiae
0.098
-
 (3R,3'R)-dimethylspermine pH 7.4, 25°C Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome
-
 Saccharomyces cerevisiae 5777
-

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3R,3'R)-dimethylspermine + 2 O2 + 2 H2O
-
 Saccharomyces cerevisiae putrescine + 2 3-aminobutanal + 2 H2O2
-
 ?
(3S,3'S)-dimethylspermine + 2 O2 + 2 H2O
-
 Saccharomyces cerevisiae putrescine + 2 3-aminobutanal + 2 H2O2
-
 ?
additional information Fms1 prefers (S,S)- to (R,R)-diastereoisomer, but with notably lower kcat in comparison with spermine. Fms1 is prone to aldehyde supplementation in its regioselectivity, i.e. the cleavage site of spermidine Saccharomyces cerevisiae ?
-
-
spermine + O2 + H2O
-
 Saccharomyces cerevisiae spermidine + 3-aminopropanal + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
Fms1
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.77
-
 (3R,3'R)-dimethylspermine pH 7.4, 25°C Saccharomyces cerevisiae
1.85
-
 (3S,3'S)-dimethylspermine pH 7.4, 25°C Saccharomyces cerevisiae
31.1
-
 spermine pH 7.4, 25°C Saccharomyces cerevisiae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
7.9
-
 (3R,3'R)-dimethylspermine pH 7.4, 25°C Saccharomyces cerevisiae
30
-
 (3S,3'S)-dimethylspermine pH 7.4, 25°C Saccharomyces cerevisiae
400
-
 spermine pH 7.4, 25°C Saccharomyces cerevisiae